Establishment of A Novel Plant Micropropagation System From Mature Hydroprimed Seeds Of Two Turkish Broad Bean Cultivars

Leguminous Broad bean (Vicia faba L.) is an excellent source of minerals, vitamins and proteins that makes it higly valuable food for human feed animal consumption. The plant has important characteristic to fix atmospheric Nitrogen play role establish a natural balance N in the atmosphere. It highly self-pollinated problem low variation with limited genetic pool. Moreover, previous studies identify recalcitrance broad bean. Therefore, there need repeatable micropropagation protocol could ensure increase variability overwhelm problems breeding. This system must also be able efficient gene delivery integrated conventional breeding programs through direct organogenesis. study aimed develop tissue culture on two Turkish cultivars Filiz99 Eresen87 using MS medium containing 0.05, 0.15, 0.25, 0.35, 0.45, 0.55 mg l-1 TDZ embryonic axis as explant. Maximum number 5.33 3 shoots per explant were noted cv. 0.15 TDZ. developing rooted 1 IAA after three weeks culture. plants transferred pots peat under maintained controlled greenhouse conditions acclimatization acclimatized bloomed set seeds. Present results underscore importance seed hydropriming before taking explants achieve high beans overcome recalcitrance.